Cryo-electron microscopy (cryo-EM) has advanced rapidly in recent years, enabling atomic-level structural analysis of viruses and proteins through Single Particle Analysis (SPA) workflows. These capabilities demand highly stable hardware and software systems.
To support this growing field, JEOL introduces the CRYO ARM™ 200, a cryo-TEM designed to deliver exceptional resolution and operational stability, enabling fully automated, unattended image acquisition for SPA.
Automated Specimen Exchange System
The CRYO ARM™ 200 features an autoloading specimen stage cooled with liquid nitrogen, along with a cryo-storage unit that accommodates up to 12 frozen-hydrated specimens. Liquid nitrogen is automatically supplied to both the stage and storage system as needed. The autoloader can load and retrieve up to four grids at a time without disturbing the remaining samples.
A unique specimen labeling system keeps management seamless and fully transparent to the operator, supporting beamline-style scheduling and efficient throughput.
Cold Field Emission Gun (Cold FEG)
The cold FEG generates a high-brightness electron beam with minimal energy spread, offering excellent temporal coherence. Improved spatial coherence further enhances the CRYO ARM™ 200’s resolution and contrast, outperforming other 200 kV cryo-TEM systems currently on the market.
In-Column Energy Filter (Omega Filter)
The improved in-column Omega filter enables the acquisition of both energy-filtered images and energy loss spectra. Zero-loss imaging reduces chromatic aberration, resulting in higher contrast and improved data quality.
Automated Image Acquisition Software for Single Particle Analysis
The CRYO ARM™ 200 includes JADAS, an automated software platform designed for SPA workflows. JADAS can automatically detect holes in the specimen grid and acquire high-resolution images without user intervention. The system is also fully compatible with SerialEM (developed at the University of Colorado Boulder), providing additional flexibility for automated data collection.
Hole-Free Phase Plate *1
An optional hole-free phase plate (HFPP), consisting of a thin, continuous carbon film positioned in the back focal plane of the objective lens, significantly enhances image contrast for frozen-hydrated specimens. The HFPP is manufactured using a proprietary process that ensures robust performance and affordability.
Auto Adjustment Functions *2
Auto focus, auto coma-free alignment, auto parallel-beam illumination, and other automated adjustments are available, ensuring image acquisition is consistently performed under optimal conditions.
*1: Optional unit. *2: Images acquired with the bottom mount camera are used.
Key Features
- Auto adjustment functions
- Accelerating voltage: 200 kV
- Automated image acquisition software for single particle analysis
- Automated specimen exchange system
- Cold field emission gun
- Hole-free phase plate
- In-column energy filter (Omega filter)